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. 2019 Jul 5;9:9736. doi: 10.1038/s41598-019-45841-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ChIPseq, ATACseq, and bulk RNAseq showed significant enrichment of cell cycle transcripts following induction of Etv2 in the ES/EB system. (a) Venn diagram of the overlap between genes associated with Etv2 ChIPseq peaks²⁵ and genes annotated to the cell cycle GO-classification. The significance was confirmed using the Fisher Exact Test. (b) Heat map of bulk RNAseq analyses of the previously published datasets¹⁰ using iHA-Etv2 ES/EBs showing increased expression of cell cycle genes in Dox-induced EBs relative to uninduced EBs. Note the increased expression of cell cycle genes following the induction of HA-Etv2 at both time points. (c) ATACseq analysis using iHA-Etv2 ESCs, D2 EBs, and D3 EBs following Dox treatment for 24 h. Note that there was significantly (p < 0.001) higher percentage of ATACseq peaks within the cell cycle genes as compared to background genes in the Dox-treated samples (***p < 0.001).