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. 2019 Jul 5;9:9736. doi: 10.1038/s41598-019-45841-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Etv2 is associated with cellular proliferation during embryogenesis. (a) Immunohistochemical analysis of transgenic Etv2-EYFP mouse embryos⁵ at E8.5. The boxed region is magnified and shown in the right panels. White arrowheads indicate EYFP⁺/Ki67⁺ double-positive cells. (b) Quantitative analysis of EYFP⁺ and Ki67⁺ cells in the Etv2-EYFP transgenic embryo sections. (b–d) qPCR analysis of cell cycle gene expression from zsGreen⁻ and zsGreen⁺ cells sorted from Etv2-zsGreen1-DR¹¹ EBs on D3 or D4 of differentiation. Pro-proliferative cell cycle genes Ccnd2 and Cdk7, showed increased expression in zsGreen⁺ cells, while expression of Cdkn2a, a cell cycle repressor, was decreased in zsGreen⁺ cells relative to zsGreen⁻ cells. Data are presented as mean ± SEM (n = 3 replicates; *p < 0.05).