Table 1.

Primer sequences and product sizes.

Gene	Primer sequence, forward/reverse (5′…3′)	Product size, bp
Actb (beta-actin)	gctgcgttttacaccctttc/gtttgctccaaccaactgct	218
Actg (gamma-actin)	ctggtggatctctgtgagca/tcaggagggaagaaaccaga	184
Actn1 (alpha-actinin 1)	ggtcagcagcaacctcctc/tctttctccaccttctctcca	167
Actn4 (alpha-actinin 4)	accctgaacagactcccttg/gatcgacaagcctccatctc	168
Tubb2b (beta-tubulin 2B)	ggcagcaagaagctaacgag/cgaacacgaagttgtctggc	302
Des (desmin)	gtgaagatggccttggatgt/cgggtctcaatggtcttgat	182
Kdm5b(lysine (K)-specific demethylase 5B, JARID1B)	agtggctttcctgttcgaga/aagcacatgcccacatacaa	173
Prm1(protamine 1)	atggccagataccgatgct/cgagatgctcttgaagtctgg	231
Spaca3(sperm acrosome associated 3, SLLP1)	caaggccaaggtcttcagtc/tcagcttcatgatccacagc	150
Dnmt1 (S-phase methylation)	ccggaaactcacttggacga/tttggcagctggatctctgg	90
Dnmt3A (de novo methylation)	agagcgctttgactccacat/ggaccaggaaaaacaaacga	150
Tet1 (cytosine demethylase)	gtgtgggtcgatggctctat/cttattcccaccaccgctaa	208
Tet2 (cytosine demethylase)	gttctcaacgagcaggaagg/tgagatgcggtactctgcac	185
Tet3(cytosine demethylase)	ttctatccgggaactcatgg/ccaggccaggatcaagataa	226
Hat1 (histone aminotransferase 1)	agagtgccgtggagaagaaa/tttcatcatccccaaagagc	150
Hdac1 (histone deacetylase 1,2,3,4,6,9)	ccatgaagcctcaccgaat/caaacaccggacagtcctca	226