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. 2019 Jul 5;9:9776. doi: 10.1038/s41598-019-46170-3

Figure 1.

Figure 1

Effects of miR-214 on cell proliferation and colony formation in prostate cancer cells. (A) RWPE-2, PC3, DU145, MDA-PCa-2b, and LNCaP cells were cultured for 30 h, and the relative endogenous expression of miR-214 was analyzed by RT/qPCR. (B) RWPE-2, PC3, DU145, MDA-PCa-2b cells, and LNCaP were transfected with negative control (NC) mimic miRNA or miR-214 mimic for 48 h. miR-214 expression levels were then determined by RT/qPCR. (C) RWPE-2, PC3, DU145, MDA-PCa-2b, and LNCaP cells were transfected with NC or miR-214 mimic. After 48 h, the effect of the miR-214 expression on cell viability was determined by MTT assay. (D) The time-dependent effects of miR-214 expression on cell viability were determined by MTT assay. (E) The effect of NC or miR-214 mimic on cell colony formation was determined by colony formation assay (left). The number of colonies were measured by ImageJ software. The data represent the colony count ± SEM (right). One-way and two-way ANOVA followed by Tukey’s multiple comparisons test were used to determine statistical significance for RT/qPCR and MTT assay, respectively. Results are representative of three independent experiments. *P < 0.05, **P < 0.005, ***P < 0.0005 compared with NC-transfected cells.