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. 2019 Jul 5;9:9776. doi: 10.1038/s41598-019-46170-3

Figure 5.

Figure 5

Effects of PTK6 overexpression on prostate cancer cell proliferation and colony formation. (A) Western blot analysis: PC3, MDA-PCa-2b, and LNCaP cells were co-transfected with empty vector (EV) or PTK6 plasmids as indicated for the first 24 h and then transfected with NC or miR-214 mimic for an additional 24 h. Cell lysates (40 µg) were immunoblotted with anti-PTK6 and anti-GAPDH antibodies. (B) MTT assay: PC3, MDA-PCa-2b, and LNCaP cells were transfected as indicated in (A) and after 48 h, cell proliferation was analyzed by MTT assay. (C) Cell colony formation assay: PC3, MDA-PCa-2b, and LNCaP cells were transfected with EV or PTK6 plasmids for 24 h and then transfected NC or miR-214 mimic for an additional 24 h. Cells were then trypsinized, counted, and re-plated in 6-well plates for analysis using colony formation assays. The data represent mean ± SEM. One-way ANOVA followed by Tukey’s multiple comparisons test was used to determine statistical significance for the cell viability and colony formation assays. Results are representative of three independent experiments. *P < 0.05, **P < 0.005, ***P < 0.0005 compared with NC mimic or PTK6 transfected cells.