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. 2019 Jul 5;9:9754. doi: 10.1038/s41598-019-45118-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Thermostability and 3-D structure of acetone-pre-incubated DLac. (a) Thermostability of DLac was decreased with increasing acetone concentration. Plots indicated the residual activities after 10 min of heat treatment. DLac activity after 10-min incubation at 25 °C was defined as 100%. Data were shown as mean ± SD from at least three independent repeats. (b) The secondary structure of DLac remained unchanged in acetone solution. Far-UV CD spectra were used to monitor DLac structure change in 0, 10, 30 and 50% acetone. (c) Crystals and 3-D structures of the tetragonal form of acetone-pre-incubated DLac and orthorhombic form of DLac were compared and superimposed. Four copper atoms are represented as orange spheres.