Fig. 1.

The single-factor test of the effect on gossypol of cottonseed meal by the H. armigera CYP9A12. The four different factors include initial temperature, enzymatic hydrolysis time, enzyme content, and substrate moisture. In accordance with the abscissa, the ordinate free gossypol content for concentration curve for result analysis. The optimal condition of the effect of H. armigera CYP9A12 enzyme on CSM gossypol degradation was achieved with 2.5 mL of enzyme. The enzymatic hydrolysis lasted 2 h at 35 °C, and the substrate moisture was 40%