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. 2019 Jul 5;9:98. doi: 10.1186/s13568-019-0823-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Extracted LC–MS ion chromatogram of the gossypol metabolites in negative ion mode and representative mass spectra of respective of chromatograms for quantification of gossypol and metabolites. a The control group without enzyme (blue) the free gossypol spontaneously degraded to compounds G1 (m/z 265) and G2 (m/z 293); b the recombinant of H. armigera CPR and CYP9A12 enzyme (black) was capable to degrade free gossypol by decarboxylation of G1 (m/z 265) and G2 (m/z 293) to compound G0 (m/z 209) and G0′ (m/z 249). The endogenous enzyme was as shown in blue (Chen et al. 2019)