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. 2019 Jul 5;9:98. doi: 10.1186/s13568-019-0823-4

Table 2.

Box–Behnken experimental factors and coding levels

Code Variable The coding level
− 1 0 1
A Initial temperature  °C 33 35 37
B Enzymolysis time h 1.5 2 2.5
C Enzyme additive amount mL 2.25 2.5 2.75
D Substrate moisture  % 38 40 42