Table 4.

Box–Behnken design and gossypol content

Treatment	Factor				Result
	A	B	C	D	Y mg/kg Gossypol content mg/kg
1	33	1.5	2.5	40	65.59
2	37	1.5	2.5	40	58.5
3	33	2.5	2.5	40	46.07
4	37	2.5	2.5	40	45.16
5	35	2	2.25	38	63.21
6	35	2	2.75	38	47.61
7	35	2	2.25	42	67.95
8	35	2	2.75	42	58.11
9	33	2	2.5	38	61.35
10	37	2	2.5	38	50.14
11	33	2	2.5	42	48.12
12	37	2	2.5	42	48.32
13	35	1.5	2.25	40	45.4
14	35	2.5	2.25	40	46.88
15	35	1.5	2.75	40	51.48
16	35	2.5	2.75	40	45.33
17	33	2	2.25	40	56.18
18	37	2	2.25	40	44.56
19	33	2	2.75	40	62.08
20	37	2	2.75	40	53.37
21	35	1.5	2.5	38	73.18
22	35	2.5	2.5	38	42.56
23	35	1.5	2.5	42	48.11
24	35	2.5	2.5	42	51.48
25	35	2	2.5	40	44.11
26	35	2	2.5	40	39.22
27	35	2	2.5	40	36.94
28	35	2	2.5	40	43.87
29	35	2	2.5	40	36.54

This table is the experimental results of Box–Behnken design and corresponding schemes. The gossypol content is selected as the response value Y. The independent variables A, B, C and D was corresponding to initial temperature, enzymatic hydrolysis time, enzyme content, and substrate moisture are shown in the table