Fig. 2.

Variant selection flowchart. Flowchart outlining variant selection for high-depth sequencing validation experiments. Candidate DNMs from trio exome sequencing were used to select candidate mosaic DNMs in the proband (blue) as well as ‘low level’ mosaic variants in a parent (light red) for validation; candidate inherited variants were used to select ‘high level’ mosaic variants in a parent (dark red) for ultrahigh-depth sequencing