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. 2019 Jul 5;9:9777. doi: 10.1038/s41598-019-46224-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The interpretation of metals interactions with DNA measured by PCR-HRM. (1) the double stranded DNA melts apart and releasing fluorescence dye away; (2) metal (•) interacts with DNA and displaces SYBR Green I; (3) metal induces DNA strand breaks and shortened amplicon releasing lower amount of fluorescence dye away at lower temperature in comparison to control (1); (4) metal binds with DNA formed cross-links and displaces fluorescence dye at higher temperature than control probe.