Table 2.

Percentage of early and late apoptotic and necrotic A549 or BEAS-2B cells following treatment with copper complexes measured by flow cytometry; mean of three independent experiments ± SD. IP – propidium iodide.

Substance [μM]	A549				BEAS-2B
	Normal cells (Annexin−/IP−)	Apoptosis		Necrosis (Annexin−/IP+)	Normal cells (Annexin−/IP−)	Apoptosis		Necrosis (Annexin−/IP+)
		Early (Annexin+/IP−)	Late (Annexin+/IP+)			Early (Annexin+/IP−)	Late (Annexin+/IP+)
Control	97.60 ± 0.71	1.45 ± 0.42	0.68 ± 0.25	0.30 ± 0.08	94.50 ± 1.12	3.08 ± 0.87	1.65 ± 0.95	0.78 ± 0.46
15 [Cu(iaa)2(H2O)]	97.55 ± 0.21	1.15 ± 0.35	0.80 ± 0.14	0.55 ± 0.35	92.10 ± 0.42	4.70 ± 0.99	2.45 ± 1.77	0.80 ± 0.28
15 [Cu(1-allim)4](NO3)2	97.00 ± 1.70	1.30 ± 0.42	1.45 ± 0.92	0.30 ± 0.28	94.65 ± 0.46	3.05 ± 0.46	1.25 ± 0.47	0.80 ± 0.53
15 CuCl2	98.10 ± 0.14	1.00 ± 0.01	0.65 ± 0.21	0.25 ± 0.07	92.35 ± 2.19	4.55 ± 0.92	2.30 ± 1.98	0.80 ± 0.71
30 [Cu(iaa)2(H2O)]	97.10 ± 0.14	0.95 ± 0.35	1.50 ± 0.14	0.40 ± 0.28	92.80 ± 1.27	3.60 ± 0.14	2.80 ± 1.98	0.80 ± 0.57
30 [Cu(1-allim)4](NO3)2	95.10 ± 1.98	0.45 ± 0.07	6.55 ± 1.63	1.65 ± 0.35	94.43 ± 1.21	2.73 ± 0.85	2.03 ± 0.75	1.20 ± 0.98
30 CuCl2	97.75 ± 0.64	1.00 ± 0.01	0.95 ± 0.21	0.25 ± 0.07	93.20 ± 3.68	4.00 ± 1.13	2.40 ± 2.55	0.50 ± 0.14
60 [Cu(iaa)2(H2O)]	94.75 ± 0.49	0.45 ± 0.07	3.30 ± 0.42	1.45 ± 1.06	93.70 ± 1.13	2.60 ± 0.28	2.65 ± 2.05	1.05 ± 0.64
60 [Cu(1-allim)4](NO3)2	68.05 ± 5.16	0.20 ± 0.01	26.35 ± 8.13	5.40 ± 2.97	94.40 ± 1.49	2.13 ± 0.55	2.13 ± 0.93	1.37 ± 0.83
60 CuCl2	94.75 ± 0.07	0.45 ± 0.07	3.25 ± 0.07	1.60 ± 0.14	93.55 ± 2.62	3.35 ± 0.78	2.45 ± 1.91	0.65 ± 0.07
125 [Cu(iaa)2(H2O)]	88.45 ± 2.33	0.10 ± 0.01	7.20 ± 0.42	4.20 ± 2.83	94.20 ± 0.70	2.00 ± 0.42	2.50 ± 0.14	1.25 ± 0.35
125 [Cu(1-allim)4](NO3)2	1.15 ± 1.48	0.05 ± 0.07	53.30 ± 30.91	45.50 ± 28.47	67.77 ± 5.58	3.93 ± 1.94	24.20 ± 2.98	4.10 ± 3.73
125 CuCl2	79.60 ± 1.13	0.15 ± 0.07	9.50 ± 2.69	10.75 ± 3.75	94.55 ± 1.77	2.65 ± 0.78	2.35 ± 1.20	0.55 ± 0.21
250 [Cu(iaa)2(H2O)]	17.25 ± 7.71	0.05 ± 0.07	14.72 ± 1.63	67.95 ± 6.15	94.50 ± 0.14	1.35 ± 0.21	3.05 ± 0.78	1.10 ± 0.42
250 [Cu(1-allim)4](NO3)2	0.00 ± 0.00	0.00 ± 0.00	23.45 ± 7.42	77.50 ± 6.08	7.33 ± 8.75	1.87 ± 1.77	77.97 ± 18.51	12.87 ± 6.00
250 CuCl2	9.95 ± 4.88	0.00 ± 0.00	23.50 ± 14.13	66.50 ± 10.32	94.15 ± 1.06	2.10 ± 0.71	2.75 ± 0.92	0.95 ± 0.64
500 [Cu(iaa)2(H2O)]	0.60 ± 0.28	0.00 ± 0.00	15.15 ± 5.87	84.25 ± 6.15	94.85 ± 1.63	0.70 ± 0.28	1.75 ± 0.78	2.75 ± 0.49
500 [Cu(1-allim)4](NO3)2	9.55 ± 1.34	1.90 ± 0.57	17.15 ± 8.96	71.40 ± 10.18	1.43 ± 0.76	1.90 ± 0.30	79.77 ± 14.88	16.93 ± 9.20
500 CuCl2	0.15 ± 0.07	0.00 ± 0.00	10.75 ± 0.64	89.05 ± 0.49	84.80 ± 1.41	1.30 ± 0.99	12.35 ± 1.48	1.55 ± 1.06

Bolded font when p < 0.05 in comparison to proper control (normal cells).