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. 2019 Jul 5;10:2976. doi: 10.1038/s41467-019-10881-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — mTOR levels are elevated in Fbxw7 mutants. Western blot analyses of sciatic nerve lysates indicate a 2-fold increase in mTOR protein in Dhh^cre(+):Fbxw7^fl/fl (cKO [conditional knockout]) nerves compared with littermate controls when normalized to both background and alpha-tubulin levels (a, b; p = 0.0350, unpaired t-test with Welch’s correction). mTOR mRNA is also upregulated in Dhh^cre(+):Fbxw7^fl/fl nerves (c; mTOR p = 0.0236, Eif4b p = 0.417, Eif4ebp1 p = 0.0398, Eif4ebp2 p = 0.0087, Prkca p = 0.0073; # = p < 0.1; one-way ANOVA), as are multiple mTOR targets. The gray line at y = 1 denotes control levels. For western blots: N = 5 controls, N = 5 Dhh^cre(+):Fbxw7^fl/fl at P42. For qPCR, N = 3 control and 3 Dhh^cre(+):Fbxw7^fl/fl at P21. Error bars depict S.D.