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. 2019 Jul 5;10:2999. doi: 10.1038/s41467-019-10850-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — OsSUF4 and SDG725 enhance the association of each other at target genes. a ChIP analyses at RFT1 and Hd3a promoters using antibodies against OsSUF4, SDG725, and H3K36me3 in WT, suf4Ri-1, 725Ri-1, and suf4Ri-1 725Ri-1 plants, respectively. Upper panel: schematic representation of RFT1 and Hd3a structures and fragments examined in ChIP-PCR. Values are the mean ± SD of three individual biological replicates normalized to the internal control OsUbiquitin5. Asterisks indicate significant differences between indicated genotypes and WT (Student’s t-test: *P < 0.01). b, c CRISPR/Cas9-mediated target mutagenesis of RFT1 and Hd3a, respectively. Upper panel: schematic representation of target site. Nucleotide insertion (shown in blue) resulted in an early stop codon (Red box) for both RFT1 and Hd3a. Left panel: phenotypes of single and double mutants. Right panel: heading date of related plants grown in paddy fields under LD (Shanghai) conditions (b) or SD (Sanya) conditions (c). Values are the mean ± standard deviation for 30 independent plants. Asterisks indicate significant differences between WT and mutants (Student’s t-test: *P < 0.01). Source data are provided as a Source Data file