Fig. 5.

OsSUF4 binds to a specific 7-bp DNA element. a Schematic representation of the truncated Hd3a promoters. b Mapping the OsSUF4-binding site within the Hd3a promoter. Asterisks indicate the position of the shifted bands. F, N, and C represent the purified full-length, N-terminal, or C-terminal His-OsSUF4, respectively. DNA probe: Hd3a-b, Hd3a-c, Hd3a-d, and Hd3a-e. Competitors: unlabeled probes (100-fold excess). c Left panel: wild-type (WT) and mutated (mu) DNA probes corresponding to Hd3a-e (key element is highlighted in red and mutated bases are shown in blue). d EMSA analysis using mutations of the 9-bp element 5′-TACGGAAAT-3′ within Hd3a-e and the 7-bp element 5′-CGGAAAT-3′ within RFT1. Source data are provided as a Source Data file