Fig. 6.

The 7-bp DNA element is important for OsSUF4 activation in rice. a Dual luciferase assay to test the ability of OsSUF4 to activate wild-type (WT) or mutated Hd3a and RFT1 promoters in planta. Upper panel: schematic representation of effector and reporter plasmids used in the transient transformation assay in rice protoplasts. Relative luciferase activities of each sample refer to the values normalized to their individual controls. The control for each sample was the same individual reporter construct without co-transfection with the effector construct. Values are the mean ± SD of three independent biological replicates. *Significant differences between WT and negative control (Student’s t-test: *P < 0.01). **Significant differences between WT and mutated reporters (Student’s t-test: **P < 0.01). b Relative transcripts of GUS by RT-PCR analysis in P_Hd3a::GUS, P_RFT1::GUS, P_Hd3a∆e::GUS, and P_RFT1∆e::GUS plants (Student’s t-test: *P < 0.01). c Transverse leaf section using 35-day-old transgenic rice plants of P_Hd3a::GUS, P_RFT1::GUS, P_Hd3a∆e::GUS, and P_RFT1∆e::GUS under SD conditions. M mesophyll cells, BS bundle sheath cells, P phloem, PP phloem parenchyma, V vascular bundle, X xylem, XP xylem parenchyma (Scale bars: 20 μm). Source data are provided as a Source Data file