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. 2019 Jul 5;10:2999. doi: 10.1038/s41467-019-10850-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — OsSUF4 binds to more target genes. a Venn diagram showing comparison of total genes containing the 7-bp element in the rice genome (green) and the down-regulated (over 1.5-fold) genes in the suf4Ri-1 mutant plants (yellow). b Venn diagram showing comparison of total genes enriched of H3K36me3 in the wild-type rice genome (rose) with genes containing the 7-bp element and down-regulated in suf4Ri-1 (blue). c H3K36me3 distribution pattern of the 100 genes identified in (b) as compared to the other 16,046 H3K36me3-enriched genes in WT rice. The indicated P-value was calculated by the Kolmogorov–Smirnov test. d EMSA analysis with purified His-OsSUF4N and probes in the upper table. Unlabeled probes were used as competitors (100-fold excess). Asterisks indicate the position of shifted bands. e ChIP analysis for OsSUF4 enrichment at the promoter regions of the seven genes using an antibody against OsSUF4 in WT, suf4Ri-1, and P_Ubi::HA-OsSUF4 plants. Values are the mean ± SD of three individual biological replicates normalized to the internal control OsUbiquitin5. *Represents significant differences between WT and suf4Ri-1 (Student’s t-test: *P < 0.01). **Represents significant differences between WT and P_Ubi::HA-OsSUF4 (Student’s t-test: **P < 0.01). Source data are provided as a Source Data file