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. 2019 Jul 5;9:9789. doi: 10.1038/s41598-019-46201-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-culture of monodisperse epithelial cells with fibroblasts results in a random distribution of the two cell types. (a,b) A single cell suspension of epithelial cells (GFP-16HBE cells (green)) was mixed with a single cell suspension of fibroblasts (DsRed-MRC5 cells (magenta)); the images show the cells at 0 h (a) and after 72 h (b) of culture. (c,d) A confluent layer of DsRed MRC5 cells was established (c) prior to addition of a single cell suspension of GFP-16HBE cells and culture for 72 h. (d) Nuclei are labelled with DAPI (blue). Scale bar either 200 μm (a,c) or 15 μm (b,d).