Figure 2.

The Editing Characteristic in Embryos Is Different from that in 293T Cells

(A) The editing efficiency of A6 and A8 for the COL9A2 gene in embryos was calculated. Data from three independent experiments are shown as means ± SD. (B) Two target sequences that contained the GGA or AGT PAM were edited using ABE-NG. The representative chromatograms of the Sanger sequencing of target sites from genomic DNA of HEK293T cells (top) and human tripronuclear embryos (bottom) are shown. The red star indicates the pathogenic point. (C) The A4 and A7 sites for the BCS1L-1 gene site were calculated by EditR software via Sanger sequencing of the PCR products derived from the target sites. Data are shown as the mean ± SD (n = 3). The A5 and A7 sites for the BCS1L-2 gene site were calculated with the same method. (D) The ratios of editing efficiency for different points of the three gene sites were calculated using the previous data.