Figure 3.

Analysis of ABE-Mediated Base Editing of the TTR Gene in Blastomeres

(A) Schematic illustration of the experimental procedure for analysis of ABE-mediated base editing in triploid blastomeres. A6 in the target sites was detected. Each string presents one allele in the tripronuclear embryos. The black triangle means adenine and the red triangle means guanine. The genotype was calculated via Sanger sequencing of the target sites. (B) Six embryos for the TTR gene were divided into triploid blastomeres. Each box indicates one embryo. The black triangle means adenine and the red triangle means guanine. (C) Summary of the alleles with the edited A₆ site in triploid blastomeres.