Table 1.
Summary of the Used Embryos Edited with ABE
| Serial Number of Injection | Used sgRNA | Edited Embryo |
Blastomere |
|||||
|---|---|---|---|---|---|---|---|---|
| Number | Efficiency >50% |
Efficiency >80% |
Number | 2 Edited Alleles |
3 Edited Alleles |
|||
| Number (%) | Number (%) | Number (%) | Number (%) | |||||
| 1 | SITE6 | 12 | 7 (58) | 3 (25) | NA | |||
| 2 | SITE2 | 12 | 12 (100) | 11 (92) | NA | |||
| 3 | ALDOB | 7 | 0 (0) | 0 (0) | NA | |||
| 4 | COL9A2 | 7 | 6 (86) | 4 (57) | NA | |||
| 5 | KCNJ11 | 7 | 0 (0) | 0 (0) | NA | |||
| 6 | TTR | 12 | 12 (100) | 10 (83) | NA | |||
| 7 | RPE65 | 10 | 6 (60) | 0 (0) | NA | |||
| 8 | TTR + RPE65 | TTR | 10 | 10 (100) | 9 (90) | NA | ||
| RPE65 | 10 | 9 (90) | 5 (50) | NA | ||||
| 9 | TTR | 6 | – | – | 48 | 46 (96) | 37 (77) | |
NA, not applicable.