Table 1.
Off-Target Genes Predicted by the CCTOP Algorithm
| Gene | Chr | Start | End | Strand | MM | Target Sequence | PAM | Position | Gene | Gene ID |
|---|---|---|---|---|---|---|---|---|---|---|
| OT-1 | 8 | 141103484 | 141103506 | + | 4 | TGAGCGGCAGAGTTCTCCTC | CGG | inter-genic | DENND3 | ENSG00000105339 |
| OT-2 | 19 | 11493685 | 11493707 | + | 4 | CTGACCCCAGACTTCTCCTC | AGG | intronic | MIR7974 | ENSG00000274713 |
| OT-3 | 3 | 182066120 | 182066142 | − | 3 | TTAAAGGAAGACTTCTCCTC | AGG | inter-genic | LINC01206 | ENSG00000242512 |
| OT-4 | 11 | 5234396 | 5234418 | + | 4 | TTGACAGCAGTCTTCTCCTC | AGG | exonic | HBD | ENSG00000223609 |
| OT-5 | 6 | 158475230 | 158475252 | − | 4 | GGAGGGGCAGGCTTCTCCTC | TGG | intronic | TULP4 | ENSG00000130338 |
| HBB | 11 | 5226984 | 5227006 | + | 0 | GTAACGGCAGACTTCTCCTC | AGG | exonic | HBB | ENSG00000244734 |
The prediction of potential off-targets were done using CC-TOP algorithm.25 Chr, chromosome number; MM, mismatch in guide sequence; PAM, protospacer adjacent motif.