Figure 2.

BM450697 Decreases LDLR mRNA Transcription through Displacement of Pol II and Possibly SREBP1a at the Promoter Site of LDLR

(A) A schematic of the LDLR promoter shows the position of the SREBP1 response element (SRE) and the primer sets used to amplify the select genomic regions for Pol II and SREBP1 binding. Hep 3B cells (2 million) were seeded and transfected the next day with either 10 nM 2′ fluorinated (2′F) pyrimidine BM450697 RNA or a lambda RNA control. Cells were cross-linked and processed for ChIP 48 h after transfection. (B) Pol II and (C) an SREBP1a ChIP. DNA was amplified with either the promoter-specific LDLR primer sets shown in (A) or with an off-target control primer set 8 kb upstream of the LDLR promoter site. Histograms are representative of the mean ± SEM of two independent experiments, performed in triplicate. One-way ANOVA with the post hoc Dunnett’s test was performed; **p < 0.01.