Figure 3.

siRNA Targeted Silencing of BM450697

(A) A schematic is shown depicting the tile of siRNAs developed for both the BM450697 transcript and its predicted promoter region. (B) siRNA screen targeting both BM450697 transcripts and predicted promoter in Hep G2 and Hep 3B cells. (C) siRNAs 4, 5, and p5 targeting of BM450697 resulted in activation of and a decrease in BM450697 in siRNA-treated Hep G2 and Hep 3B cells. (B and C) Data are representative of the mean ± SD of three independent experiments with triplicate treated conditions. The significance of differences between two groups was determined by non-parametric one-way ANOVA with the post hoc Dunn’s test (B) or by two-way ANOVA with the post hoc Tukey’s test or Student’s t test (C). *p < 0.05, **p < 0.01, and ***p < 0.0001.