Supplementary Figure S1.

Normalized gene expression values. The cycle threshold (Ct) values were normalized using the delta–delta Ct method, and bold gene signatures were retained for the survival analysis. Light gray gene signatures, which cannot distinguish between positive samples (blue) and negative controls (black), were omitted. Each dot represents one analyzed gene expression datum from a patient with metastatic castration-resistant prostate cancer or a healthy control. The dashed and dotted lines represent grouping thresholds determined by k-means clustering used to test association between sample source and grouping. The y-axis shows the normalized gene expression values.