Figure 2.

Sclerostin (Sost) expression is differentially regulated after glucocorticoid treatment ex vivo and in vitro. Real-time PCR analysis of Sost mRNA expression in murine femoral bone tissue of treated (vehicle (CO) or prednisolone (GC)) C57BL/6 JRi mice (A, B, C and D) and in murine bone marrow stromal cells (BMSC) treated with two different doses of dexamethasone (DEX; 0.1 and 1 µM; F, G, H and I) for 48 h. Figure 2E and J show normalized and pooled data of experiments A, B, C, D and F, G, H, I, respectively. n = 1–7; *P  < 0.05 vs CO.