Table 5.
Primers used to detect ESBL-resistance genes and genotypes in broiler farm isolates
| Target gene | Primer | Sequence (5 → 3) | Annealing Temp (°C) | Size (bp) | Ref |
|---|---|---|---|---|---|
| bla CTX-M |
CTX-M-F CTX-M-R |
ATGTGCAGYACCAGTAARGTKATGGC TGGGTRAARTARGTSACCAGAAYSAGCGG |
55 | 592 | [29] |
| bla CTX-M-1group |
CTX-M-1-F CTX-M-1-R |
GGTTAAAAAATCACTGCGTC TTACAAACCGTYGGTGACGA |
50 | 873 | [29] |
| bla CTX-M-15 |
CTX-M-15-F CTX-M-15-R |
CACACGTGGAATTTAGGGACT GCCGTCTAAGGCGATAAACA |
50 | 995 | [30] |
| bla CTX-M-2group |
CTX-M-2-F CTX-M-2-R |
ATGATGACTCAGAGCATTCGCCGC TCAGAAACCGTGGGTTACGATTTT |
56 | 876 | [31] |
| bla CTX-M-8group |
CTX-M-8-F CTX-M-8-R |
TGATGAGACATCGCGTTAAG TAACCGTCGGTGACGATTTT |
52 | 666 | [32] |
| bla CTX-M-9group |
CTX-M-9-F CTX-M-9-R |
GTGACAAAGAGAGTGCAACGG ATGATTCTCGCCGCTGAAGCC |
55 | 856 | [33] |
| bla CTX-M-25group |
CTX-M-25-F CTX-M-25-R |
GCACGATGACATTCGGG AACCCACGATGTGGGTAGC |
52 | 327 | [34] |
| bla TEM |
TEM-F TEM-R |
TTGGGTGCACGAGTGGGTTA TAATTGTTGCCGGGAAGCTA |
55 | 506 | [35] |
| bla SHV |
SHV-F SHV-R |
TCGGGCCGCGTAGGCATGAT AGCAGGGCGACAATCCCGCG |
52 | 628 | [35] |