Fig. 4.

Modern and historic calculus microbial community profiles overlap. a Principal component analysis (PCA) does not cluster modern and historic healthy site calculus distinctly. b Historic and modern healthy site calculus contain sufficient discriminatory taxa for distinct clustering by sparse partial least squares-discriminant analysis (sPLS-DA). c PCA does not cluster modern and historic disease site calculus distinctly. d Historic and modern disease site calculus contain sufficient discriminatory taxa for distinct clustering by sPLS-DA. e Modern healthy and disease site calculus microbial profiles are not distinctly different and are not separated by PCA. f Modern healthy and disease site calculus contain sufficient discriminatory taxa for distinct clustering by sPLS-DA. g Historic healthy and disease site calculus microbial profiles are not distinctly different and are not separated by PCA. h Modern healthy and disease site calculus do not contain sufficient discriminatory taxa for distinct clustering by sPLS-DA. Ellipses indicate 95% confidence intervals