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. 2019 Jul 5;19:295. doi: 10.1186/s12870-019-1911-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunofluorescence microscopy of protein bodies in the subaleurone cells of the wild type and gpa6 mutant. a to f Immunofluorescence microscopy images of storage proteins in wild-type (a-c) and gpa6 (d-f) 12 DAF seeds. a, d Secondary antibodies conjugated with Alexa fluor 488 (green) were used to trace the antigens recognized by the anti-glutelin antibodies. (b, e) Secondary antibodies conjugated with Alexa fluor 555 (red) were used to trace the antigens recognized by the anti-prolamin antibodies. (c, f) Merged images. White arrows in (f) indicate the PMB structures. Bars = 10 μm (a-f). (g) and (h) Statistical analysis of the diameters of PBIIs (g) and PBIs (h). Values are means ± SD. **P < 0.01 (n > 350, Student’s t test)