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. 2019 Jul 5;19:295. doi: 10.1186/s12870-019-1911-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Map-based cloning of GPA6. a Fine mapping of the GPA6 locus. The molecular markers and the number of recombinants are shown. b Gene structure and the mutation site in Os09g0286400. Os09g0286400 comprises 22 exons (closed boxes) and 21 introns (lines). ATG and TGA represent the start and stop codons, respectively. A 7 bp deletion in the sixth exon of Os09g0286400 in gpa6. c-f The Os09g0286400 ORF under the control of ubiquitin promoter rescues the grain appearance (c), the storage protein composition pattern (d), the ultrastructures of endosperm cells in the wild type (e) and L1 (f). L1 to L3 denote the grains from three independent T1 transgenic lines. Red arrows in (d) indicate the 57-kD proglutelins. Bars = 1 mm in (c). Bars = 2 μm in (e) and (f)