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. 2019 Jul 5;19:295. doi: 10.1186/s12870-019-1911-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Phylogenetic analyses and spatial expression patterns of OsNHX5. a A neighbor-joining tree of OsNHX5 and its homologs. The tree was constructed using MEGA and bootstrapped with 1000 replicates. b Wild-type expression levels of OsNHX5 and OsNHX6 in various organs and different developmental stages of endosperm. R, root; S, stem; L, leaf; LS, leaf sheath; P, panicle; DAF, days after flowering. Actin1 was used as an internal control. For each RNA sample, three technical replicates were performed. Values are means ± SD