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. 2019 Jul 5;19:295. doi: 10.1186/s12870-019-1911-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — OsNHX5 regulates the pH of Golgi, TGN and PVC. a In vivo calibration curve of pH. pH calibration was achieved by equilibrating intracellular pH with 10 μM nigericin, 60 mM KCl, and 10 mM MES/HEPES Bis-Tris-propane, pH 5.0 to 8.0. (mean ± SD; n ≥ 25 protoplasts). b and c Representative pseudocolored images of PRpHluorin-BP80 (Y612A) in wild-type (b) or gpa6 (c) protoplasts. Bars = 5 μm. d pH of the Golgi, TGN and PVC. (mean ± SD; n ≥ 25 protoplasts; **P < 0.01; Student’s t test)