Fig. 2.

Malignancy of individual clones and tumor heterogeneity. a-c Tumor growth over 34 days after orthotopic injection of MDA-MB-231, GFP C3, mKO E10, or Sapphire D7 clonal cell lines and an equal mix of the previous cell lines (mix): (a) volume relative to MDA-MB-231 tumors (n = 12 per group), (b) macroscopic images of the tumors (scale bar = 0.5 cm) and (c) hematoxylin/eosin stains of tumor sections (scale bar = 50 μm) (three different animals per cell line: ID1, ID2, ID3). d-e Tumor heterogeneity: (d) representative fluorescent images (scale bar = 50 μm) of three independent sections of tumor formed by MDA-MB-231 parental and GFP C3, mKO E10 and Sapphire clonal cell lines (mix) and (e) quantification of tumor composition formed by the clonal cell lines (n = 6). Significant differences were determined using unpaired t-test with Welch’s correction. Asterisks indicate significant differences when P-values are < 0.05 (*), < 0.01 (**), and < 0.001 (***)