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. 2019 Jul 5;16:137. doi: 10.1186/s12974-019-1520-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — General average level of spontaneous and VSV-induced cytokine production by PBLs of AD patients after PRP treatment (t = 1). Freshly isolated PBLs (1 × 10⁶/ml) from blood samples of AD patients after PRP treatment were divided into two groups. One was cultured uninfected and second was infected with VSV (10² TCID₅₀/ml). After 40 min of VSV adsorption at RT, PBLs were washed with culture medium. All cells were next incubated at 37 °C for 24 h. Samples of medium above cultured uninfected (spontaneous cytokine production) and VSV-infected PBLs were collected for cytokine measurement with ELISA. Results has been analyzed with the Student t test for pairs