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. 2019 Jul 5;10:198. doi: 10.1186/s13287-019-1299-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. b Oct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01