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. 2019 Jul 5;10:198. doi: 10.1186/s13287-019-1299-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Pathological ovaries exhibited a decline in Hh pathway activity. a HE staining of a normal ovary and POF ovary. Bar is 200 μm. b The ratio of atretic follicles of normal and POF ovaries. c IHC shows that the Mvh-positive follicles and FGSCs dramatically disappeared in the POF model; green arrows are FGSCs, and black arrow represents primordial follicles, the scale is 100 μm. d–f mRNA and protein expression levels of Mvh, Oct4, Gli1, Ptch1, and Cyclin D1. *p < 0.05 and **p < 0.01