Table 1.

Primer sequences used for quantitative real-time PCR

Genes	Primer Sequence(5′-3′)	Gene number
Gli1	Forward: CCCAATACATGCTGGTGGTG Reverse: GCAACCTTCTTGCTCACACA	NM_010296.2
PTCH1	Forward: TGTGCGCTGTCTTCCTTCTG Reverse: ACGGCACTGAGCTTGATTC	NM_001328514.1
CyclinD1	Forward: GATGAGGAAGAGTTGCTAGAAGAG Reverse: TCGTCAGCCAATCGGTAGTAG	NM_007631.2
Mvh	Forward: GTGTATTATTGTAGCACCAACTCG Reverse: CACCCTTGTACTATCTGTCGAACT	NM_001145885.1
Oct-4	Forward: AGCTGCTGAAGCAGAAGAGG Reverse: GGTTCTCATTGTTGTCGGCT	NM_013633.3
Stella	Forward: CCCAATGAAGGACCCTGAAAC Reverse: AATGGCTCACTGTCCCGTTCA	NM_139218.1
Fragilis	Forward: CTGGTCCCTGTTCAATACACTCTT Reverse: CAGTCACATCACCCACCATCTT	NM_025378.2
Nanog	Forward: TCTCCTCGCCCTTCCTCTGA Reverse: TCCGCATCTTCTGCTTCCTG	NM_001289828.1
Bcl-2	Forward: GAACTGGGGGAGGATTGTGG Reverse: GCATGCTGGGGCCATATAGT	NM_009741.5
Bax	Forward: GAACCATCATGGGCTGGACA Reverse: AGCCACCCTGGTCTTGGAT	NM_007527.3
P16	Forward: AAGGCTTTGAGAGCCCATCT Reverse: CACTTCTGTGAACGGTGTCC	NM_001040654.1
P21	Forward: TAGAGAGCTGGATGCCACTG Reverse: CCTGGCATAGCCAGTGTAGA	NM_001111099.2
GAPDH	Forward: CGTGCCGCCTGGAGAAACCTG Reverse: AGAGTGGGAGTTGCTGTTGAAGTCG	NM_001289726.1