Table 1.
Testicular morphometric and stereological findings in control group (C), healthy animals that received troxerutin (T), diabetic animals (DM), diabetic animals that received troxerutin (DT) and diabetic animals that received insulin (DI) (n=6 in each group)
|
Study groups
|
Variable | ||||
|---|---|---|---|---|---|
| DI | DT | DM | T | C | |
| 1.07±0.10 | 1.27±0.10 | 1.14±0.20 | 1.05±0.10 | 0.91±.05 | Testes relative weight (%) |
| 1.15±0.10 | 0.97±0.10 | 0.95±0.10 | 1.10±0.10 | 0.95±0.5 | Left testicle weight (g) |
| 1.77±0.05 | 1.65±0.10 | 1.55±0.04 | 1.71±0.10 | 1.71±0.04 | Left testicle length (cm) |
| 0.94±0.02* | 0.82±0.04 | 0.79±0.01 | 0.84±0.01 | 0.89±0.04 | Left testicle width (cm) |
| 1.05±0.01 | 0.96±0.02 | 0.95±0.13 | 1.07±0.11 | 0.99±0.02 | Left testicle height (cm) |
| 1200.00±69.20 | 1088.00±51.61 | 982.00±34.40* | 1212.00±36.00 | 1110.00±25.10 | Left testicle volume (mm3) |
| 1006.30±61.00!!! | 903.36±45.20!! | 682.78±19.00+++*** | 1089.67±33.15 | 983.98±28.50 | Seminiferous tubule volume (mm3) |
| 788.62±62.30!!! | 693.44±42.30!!! | 356.44±7.92+++*** | 894.92±31.50 | 778.60±37.6 | Germinal epithelium volume (mm3) |
| 217.68±6.35!!! | 209.92±4.25!!! | 326.34±14.90+++*** | 194.75±5.60 | 205.38±10.80 | Lumen volume (mm3) |
| 29.38±4.54!! | 25.96±2.50!! | 45.16±2.80+++*** | 18.25±2.05 | 20.08±1.72 | Capsule volume (mm3) |
| 164.32±6.12++**!!! | 158.68±6.84++**!!! | 254.06±14.42+++*** | 104.08±3.30 | 105.94±5.80 | Interstitial tissue volume (mm3) |
| 17.46±0.80! | 17.38±0.85! | 14.53±0.60* | 17.64±0.30 | 16.86±0.50 | Seminiferous tubule length (m) |
| 423.00±1.22++**!!! | 422.00±1.22++**!!! | 407.00±2.00+++*** | 432.00±1.22 | 435.00±3.53 | Seminiferous tubule diameter (µm) |
| 120.00±2.23+++***!! | 119.00±1.90+++***! | 111.00±1.00+++*** | 139.00±1.00 | 138.00±1.22 | Germ cell layer height (µm) |
| 36.00±2.07+++*** | 34.70±1.50+++*** | 29.46±1.03+++*** | 59.39±2.82 | 59.86±0.83 | No. of spermatogonia (×106) |
| 170.94±7.41+++***!!! | 155.34±6.30+++***! | 127.74±6.10+++*** | 217.68±4.25 | 217.40±3.75 | No. of spermatocyte (×106) |
| 279.44±12.60+++*** | 275.20±8.40+++*** | 253.68±11.72+++*** | 376.98±6.84 | 377.34±12.65 | No. of round spermatid (×106) |
| 306.88±16.60+++***! | 274.92±6.50+++*** | 251.80±14.24+++*** | 426.26±10.20 | 430.66±12.60 | No. of elongated spermatid (×106) |
| 24.44±1.34++**!!! | 24.62±0.40++**!!! | 15.49±0.42+++*** | 31.27±1.20 | 32.00±1.71 | No. of Sertoli cells (×106) |
| 17.46±0.80++**!!! | 17.50±0.60++**!!! | 11.99±0.64+++*** | 20.61±0.80 | 21.09±0.50 | No. of Leydig cells (×106) |
No; number.
++
; p<0.01 and
+++
; p<0.001 show statistical differences between control and different groups.
*
; p<0.05,
**
; p<0.01,
***
and p<0.001 show statistical differences between troxerutin and different groups.
!
p<0.05,
!!
; p<0.01,
iii
;p<0.001 show statistical differences between DT and DI with DM group.