Figure 1.

Global and local changes in DNA 5hmC in a murine model of melanoma. A, Schematic representation of the transgene. Mice expressing the Nras oncogene are hyperpigmented (nevus-like) and develop melanoma (left, photo of a mouse bearing a skin melanoma; right, H&E of skin and melanoma sections). B, Global DNA 5hmC content of melanoma tumours (T; n = 3) and control nevi (N; n = 3), measured by mass spectrometry. Data, means±SD. Statistical evaluation with Student unpaired two-tailed t test gave P = 0.003 indicated by *. C, Hierarchical clustering of nevi and melanoma samples based on 5hmC-containing DNA regions (nevi n = 3, tumors n = 3). D, Proportional distribution of differentially hydroxymethylated regions (DhmR) along the genome (color bars) compared with expected (gray). E, Numbers of differentially hydroxymethylated regions (hypo or hyper) and their localization (prom, promoter; GB, gene body). F, Hierarchical clustering based on the expression of genes determined by RNA-seq. G, Number of transcripts showing statistically differential expression between melanoma tumors (T) and nevi (N). H, Area-proportional Venn diagram representation of DhmR and differentially expressed genes.