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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Allergy Clin Immunol. 2019 Feb 6;144(1):236–253. doi: 10.1016/j.jaci.2019.01.033

Figure 4: Pik3cd GOF mutations enhance the formation of memory, Tfh and Treg cells.

Figure 4:

Spleens from young (8–12 weeks) and aged (30–40 weeks) WT and Pik3cd GOF mice were stained to identify different CD4+ T cell populations by flow cytometric analysis. (A) Frequency of total CD4+ T cells. (B) Percentages of naïve (CD44loCD62Lhi), central memory (CD44hiCD62Lhi) or effector memory (CD44hiCD62Llo) CD4+ T cells. (C) Contour plots show representative staining of CXCR5 versus PD-1 on CD4+ T cells in aged mice. (D) Tfh cell (CXCR5hiPD-1hi) frequencies. (E) Contour plots show representative staining of FoxP3 versus CD25 on CD4+ T cells in aged mice. (F) The percentage of FoxP3+ T cells (CD25+ or CD25−). All graphs show mean ± SEM, n=7–11. Significant differences were determined by unpaired Students t-tests, **p<0.01; ****p<0.0001.