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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Differentiation. 2019 Mar 22;107:1–10. doi: 10.1016/j.diff.2019.03.002

Figure 1. Hes1 expression in embryonic urogenital development.

Figure 1.

(A) Illustration of transgenic alleles in Rosa26mTmG/+:Hes1CreERT2/+ (R26mTmG/+: Hes1CreERT2/+) mice. CreERT2: tamoxifeninducible Cre recombinase/estrogen receptor fusion protein, TM: tamoxifen, mG: membrane-bound GFP, mT: membrane-bound tdTomato, pA: polyadenylation-coding region, pCA: chicken beta-actin promoter with cytomegalovirus enhancer. (B) Schematic of experimental timeline for TM induction of Cre activity and analysis of urogenital tissues. DOC: day of conception. (C) mTmG reporter expression in male urogenital sinus (UGS); TM induction at embryonic day 13.5 (E13.5); analysis at E18.5. UGE: urogenital sinus epithelium, UGM: urogenital sinus mesenchyme. (D) mTmG assay on male UGS, TM induction at E15.5; analysis at E18.5. (E) Quantification of TM-induced GFP expression at E13.5 or E15.5 in E18.5 urogenital epithelium (UGE). Please also see Supplemental Table 1 in the Supplemental data file. (F) Coimmunofluorescent staining (Co-IF) for E-cadherin and GFP in E18.5-male UGS; TM induction at E13.5, (G) Co-IF for androgen receptor (AR) and GFP in E18.5 UGS; TM induction at E13.5. (H) Co-IF for E-cadherin and GFP in E18.5-male UGS; TM induction at E15.5. (I) Co-IF for AR and GFP in E18.5-male UGS; TM induction at E15.5. Dashed lines delineate UGE and UGM. Arrows indicate GFP+ cells in UGE area. Scale bars: 100 μm, or 25 μm in magnified views. Results are mean +/− SD of at least three independent samples per group. *P<0.05.