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. Author manuscript; available in PMC: 2020 Aug 13.
Published in final edited form as: Biochem Biophys Res Commun. 2019 Jun 10;516(1):50–56. doi: 10.1016/j.bbrc.2019.05.190

Figure 2: Nucleotide metabolic enzymes localize at the lamellipodial membrane.

Figure 2:

Purine nucleotide metabolism pathway. De novo enzymes (orange), ATP biosynthetic branch (green), and GTP biosynthetic branch (blue) (A). Fluorescence imaging for GTP biosynthetic enzymes GMPS (mCherry-tagged, 786-o), GUK1 (Myc, Caki-1), and NDPK-A (antibody, Caki-1) (B). Immunofluorescence staining for de novo enzymes PRPS1 (Myc, Caki-1), FGAMS (GFP, Caki-1), PAICS (GFP, Caki-1), ADSL (GFP, 786-o), and ATIC (GFP, Caki-1) (C). Insets of leading edge in pseudocolor shown below. Scale bars indicate 10 μm. Asterisk (*) indicates enzymes involved in multiple pathways. PRPS: phosphoribosyl pyrophosphate synthetase. PPAT: phosphoribosyl pyrophosphate amidotransferase. GART: phosphoribosylglycinamide formyltransferase. FGAMS: phosphoribosyl formylglycinamidine synthase. PAICS: phosphoribosyl aminoimidazole succinocarboxamide synthetase. ADSL: adenylosuccinate lyase. ATIC: 5-amino-4-imidazolecarboxamide ribonucleotide transformylase/IMP cyclohydrolase. ADSS: adenylosuccinate synthase. AK: adenylate kinase. IMPDH: inosine-5′-monophosphate dehydrogenase. GMPS: GMP synthase. GUK1: guanylate kinase 1. NDPK: nucleoside-diphosphate kinase. GAPDH: glyceraldehyde 3-phosphate dehydrogenase.