(A). Western Blot analysis of slices exposed to vehicle or insulin (10 nM) showing the amounts of phopho-AKT, total AKT, phospho- ERK, and total ERK. (B) Quantifications of the relative amount of phospho-AKT (relative to total AKT) with control or insulin. **P = 0.0003 unpaired t- test with Welch’s correction due to unequal standard deviation, F=534.1. DFn=3, Dfd = 3. (C) Quantifications of the relative amount of phospho-ERK (relative to total ERK) with control or insulin. P = .3622 with unpaired t test, t = 0.9860, df = 6. Bars are mean and s.e.m, n=4 mice per group,