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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Neuroscience. 2019 May 28;411:237–254. doi: 10.1016/j.neuroscience.2019.05.033

Fig. 4. Western blotting analysis of AKT and ERK1/2 activation in isolated hippocampal slices after 10 nM insulin stimulation.

Fig. 4

(A). Western Blot analysis of slices exposed to vehicle or insulin (10 nM) showing the amounts of phopho-AKT, total AKT, phospho- ERK, and total ERK. (B) Quantifications of the relative amount of phospho-AKT (relative to total AKT) with control or insulin. **P = 0.0003 unpaired t- test with Welch’s correction due to unequal standard deviation, F=534.1. DFn=3, Dfd = 3. (C) Quantifications of the relative amount of phospho-ERK (relative to total ERK) with control or insulin. P = .3622 with unpaired t test, t = 0.9860, df = 6. Bars are mean and s.e.m, n=4 mice per group,