Body temperature following subcutaneous administration of the anesthetic drug
combinations. (a) 0.75MBA40 (0.75 mg/kg medetomidine, 5 mg/kg butorphanol, and 40
mg/kg alfaxalone). (b) 0.75MBA30 (0.75 mg/kg medetomidine, 5 mg/kg butorphanol, and
30 mg/kg alfaxalone). (c) 0.5MBA30 (0.5 mg/kg medetomidine, 5 mg/kg butorphanol, and
30 mg/kg alfaxalone). (d) MMB (0.3 mg/kg medetomidine, 4 mg/kg midazolam, and 5
mg/kg butorphanol). Green circles: ICR mice; orange triangles: C57BL/6 mice; blue
squares: BALB/c mice. Left panels: males; right panels: females. Body temperature of
37°C is shown by the red lines. Results are presented as the mean ± SEM of five
mice. Statistical analysis was performed using the Kruskal-Wallis test with
Steel-Dwass multiple pairwise post hoc comparisons. *Significant difference
(P<0.05) in ICR vs. BALB/c mice, †Significant difference
(P<0.05) in C57BL/6 vs. BALB/c mice. ‡Significant difference
(P<0.05) in ICR vs. C57BL/6 mice.