Table 2. Characterization of 16S rRNA methylases-positive isolates from dogs and their transconjugants.
| Strain name | Bacterial species | Year | MIC (µg/ml)a) | Antibiotic resistance genes | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GM(16)b) | AMK(64)b) | NEO(32)c) | APR(64)c) | ABK(32)d) | SM(64)c) | ABPC(32)b) | TET(16)b) | 16S rRNA methylases | Aminoglycoside-modifying enzyme | Beta-lactamase genes | Tetracycline resistance gene | ||||
| 16S rRNA methylase-positive isolates | RGU-60 | Escherichia coli | 2005 | >256 | >256 | >256 | 16 | >128 | >128 | >128 | 64 | rmtB | aphA1, strA, strB | blaTEM | tetA |
| RGU-78 | E. coli | 2005 | >256 | >256 | 8 | 16 | >128 | >128 | >128 | 1 | rmtB | strA, strB | blaTEM | ||
| KL39 | Klebsiella pneumoniae | 2015 | >256 | >256 | 256 | 8 | >128 | 64 | >128 | 512 | armA | aphA1, strA, strB | blaTEM, blaSHV, blaDHA | tetA | |
| Recipient | DH5α | E. coli | 0.25 | 0.5 | 2 | 1 | 1 | 0.25 | 2 | 2 | |||||
| Transconjugants | TC-RGU-60–1 | >256 | >256 | 1 | 1 | >128 | >128 | >128 | 1 | rmtB | strA, strB | blaTEM | |||
| TC-RGU-60–2 | >256 | >256 | >256 | 16 | >128 | >128 | >128 | 64 | rmtB | aphA1, strA, strB | blaTEM | tetA | |||
| TC-RGU-60–3 | >256 | >256 | >256 | 16 | >128 | >128 | >128 | 2 | rmtB | aphA1, strA, strB | blaTEM | ||||
| TC-RGU-78–1 | >256 | >256 | 1 | 1 | >128 | >128 | >128 | 1 | rmtB | strA, strB | |||||
a) Values in parentheses indicate breakpoints. Bold type indicates resistance to individual antibiotics. b) Clinical Laboratory Standards Institute (CLSI) breakpoints [2]. c) Previously reported breakpoints [10]. d) Reference from CLSI breakpoints for amikacin. ABK; arbekacin, ABPC; ampicillin, AMK; amikacin, APR; apramycin, GM; gentamicin, MIC; minimum inhibitory concentration, NEO; neomycin, SM; streptomycin, TET; tetracycline.