Temporal Progression of NCALD Protein Levels in Mice Injected with SMN-ASO at P1 and Ctrl-ASO or Ncald-ASO3 at P2
Western blot analysis of NCALD and SMN levels in the spinal cord and the brain at (A) P21 and (B) 3 months after injection with respective ASOs (n = 3 per genotype and age). NCALD protein levels normalized to ACTB (loading control) are shown on the right side. NCALD levels are significantly decreased in both spinal cord and brain of HET and SMA animals co-injected with SMN-ASO and Ncald-ASO3 at P21 (A) but not at 3 months (B). Color legend for graphs is displayed at the bottom. For statistics, ordinary one-way ANOVA was applied with Tukey posthoc test for multiple comparisons; n.s., not significant, ∗∗p < 0.01, ∗∗∗p < 0.001.