Figure 5.

Fibroblasts Demonstrate Decreased RINT1 Protein, but Not NBAS or p31

(A) There is a significant difference in RINT1 protein levels among the genetic variants (control [Ctrl], proband 1 [Pr 1], or proband 3 [Pr 3]) (single factor effect; F_2,12 = 98.17, p = 3.65e−8). There is no significant effect of temperature (37°C versus 40°C) (single factor effect; F_1,12 = 4.73, p = 0.05). There is no significant effect on RINT1 protein levels of the interaction between the genetic variants (Ctrl, Pr 1, or Pr 3) and temperature (37°C or 40°C) (F_2,12 = 1.30, p = 0.31).

(B and C) No statistically significant reduction in NBAS protein (B) or p31 protein (C) levels compared to control were observed. 2-way analysis of variance (2-way ANOVA) from three independent experiments each. ^∗p < 0.05.