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Overlay of ion exchange chromatograms showing the separation of singly sulfo-Cy5 labeled FUD and 10–40 kDa PEG-FUD from the unreacted and multiply labeled peptides. The collected fraction containing the singly labeled drug is indicated with arrows. An anionic exchanger in conjunction with 20 mM Tris (pH 8) A side and 1 M NaCl in 20 mM Tris Buffer (pH 8) B side mobile phases were used to elute the peptides
