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Isothermal titration calorimetry (ITC) was used to determine the binding affinity (Kd) and other binding parameters of the interaction between the sulfo-Cy5 labeled peptide and human plasma fibronectin. Shown here are isotherms and thermographs of experiments involving a FUD-Cy5 or b 20 kDa PEG-FUD-Cy5 interaction with FN. All experiments were performed in duplicates using pH 7.4 PBS at 25 °C
